Quick answer: to reconstitute a lyophilized peptide, divide the vial amount (mg) by the volume of bacteriostatic water (mL) you add to get the concentration in mg/mL. Enter your vial size and diluent below and this calculator returns the concentration, the exact volume to measure for any target amount, that volume in U-100 insulin-syringe units, and how many measurements a vial yields. For in-vitro laboratory research use only.
Figures are calculated math for laboratory measurement and aliquoting only. Bacteriostatic water does not change the mass of peptide in the vial — it only sets the concentration. This tool is not medical, veterinary, or dosing guidance.
All materials referenced are lyophilized reference standards supplied for in-vitro laboratory research only — not for human or veterinary use. This calculator performs concentration and volume math for laboratory measurement; it is not a recommendation of any amount for any use.
Note the lyophilized peptide amount in milligrams printed on the label (for example 10 mg).
Choose how much bacteriostatic water to add. More water = lower concentration and a larger, easier-to-measure volume.
Concentration (mg/mL) = vial mg ÷ water mL. Multiply by 1000 for mcg/mL.
Volume (mL) = target amount ÷ concentration. Multiply by 100 for U-100 syringe units.
Every lot ships with a third-party Certificate of Analysis — HPLC purity and LAL endotoxin, traceable by the lot number on the vial. Browse the COA archive →
There is no single correct amount — the water only sets the concentration, not the total peptide. A common approach is to pick a diluent volume that makes your target measurement land on an easy-to-read syringe mark. For example, adding 2 mL to a 10 mg vial gives 5 mg/mL, so 250 mcg is 0.05 mL (5 units on a U-100 syringe). Use the calculator above to test different volumes.
A standard U-100 insulin syringe has 100 units per 1 mL. Multiply the volume in milliliters by 100 to get units. So 0.05 mL = 5 units, and 0.10 mL = 10 units.
No. Reconstitution only dissolves the lyophilized powder into solution — the mass of peptide in the vial is fixed. Adding more water lowers the concentration (mg/mL) and increases the volume you measure for the same amount; it does not add or remove peptide.
Divide the total vial amount by your target amount per measurement. A 10 mg (10,000 mcg) vial measured at 250 mcg yields 40 measurements. The diluent volume does not change this — only the vial size and target amount do.
Bacteriostatic water is sterile water containing ~0.9% benzyl alcohol, which inhibits bacterial growth and allows a reconstituted vial to be stored and measured from over multiple sessions. Sterile water has no preservative and is intended for single measurement. For research reconstitution where a vial is accessed more than once, bacteriostatic water is typically preferred.
No. All products are lyophilized reference standards for in-vitro laboratory research only. This tool performs concentration and volume math for laboratory measurement and aliquoting; it does not recommend any amount for any human or animal use.